Due to fold and fault structures of Anhua syncline, groundwater runoff and outcrop characteristics have certain rules, and most of the dew point springs are used as living water in nearby villages. In this paper, hydrogeological conditions , chemical composition of groundwater, and hydrogen and oxygen isotopic data in the study area were analyzed, and the water balance method, hydrogeochemical reverse simulation and other means were adopted. It was found that there were three kinds of groundwater runoff modes co-existing in this area: groundwater runoff patterns along the strip of karst, fault conducting groundwater runoff patterns around the syncline axis and groundwater runoff patterns along the fault fracture zone. The results revealed the rules of runoff and discharge of groundwater in the area, therefore providing significant reference for the development, utilization and protection of groundwater resources there.